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Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 36 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008067
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416629)
Target mass (kDa)
26.1, 23.3, 20.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA008067
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA008067
Antigen
Recombinant protein fragment
Length (aa)
101
Antigen sequence
VLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGY
YDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSST
A
