VEZF1 - Antibodies - The Human Protein Atlas

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VEZF1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027520

Antibody HPA048315

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027520

HPA048315

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

PATZ1 - ENSG00000100105 [86%]
MAZ - ENSG00000103495 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA027520

Antibody HPA048315

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027520

Antibody HPA048315

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:7

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027520

Antibody HPA048315

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human kidney shows weak nuclear positivity in cells i tubules.

Immunohistochemical staining of human kidney shows nuclear and cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 30 cell types

RNA: detected in 37 tissues
Protein: detected in 64 cell types

Retrieval

HIER pH6

Antibody dilution

1:10

1:150

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027520

Antibody HPA048315

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.9, 55.8, 37.3

74.1, 69.1, 58, 57.6, 56.9, 55.8, 51.1, 48.6, 46.4, 37.3, 26.8, 22.2

Antibody dilution

1:250

1:180

PROTEIN ARRAY

Antibody HPA027520

Antibody HPA048315

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA027520

Antibody HPA048315

Antigen

Recombinant protein fragment

Length (aa)

140

Antigen sequence

RLWEEAVKARKKEAANLCQTSTAATTPVTLTTPFSITSSVSSGTMSNPVT
VAAAMSMRSPVNVSSAVNITSPMNIGHPVTITSPLSMTSPLTLTTPVNLP
TPVTAPVNIAHPVTITSPMNLPTPMTLAAPLNIAMRPVES

SDEKPYQCPVCQQRFKRKDRMSYHVRSH

Matching transcripts

VEZF1-001 - ENSP00000462337 [100%]
VEZF1-002 - ENSP00000464687 [100%]
VEZF1-005 - ENSP00000258963 [100%]

VEZF1-001 - ENSP00000462337 [82%]
VEZF1-002 - ENSP00000464687 [82%]
VEZF1-005 - ENSP00000258963 [82%]

Other gene match

PATZ1 - ENSG00000100105 [86%]
MAZ - ENSG00000103495 [100%]

ANTIGEN VIEW

VEZF1-001	VEZF1-002	VEZF1-005

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.