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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
Antibody dilution
1:200
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008965
Antibody CAB009445
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human colon shows strong immunoreactivity in erythrocytes present in blood vessel and moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows selective positivity in erythrocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 61 cell types
RNA: detected in 37 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008965
Antibody CAB009445
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419537)
Target mass (kDa)
13.8, 11.9, 11.5
13.8, 11.9, 11.5
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA008965
Antibody CAB009445
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
