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Immunohistochemical staining of human stomach, upper shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of plasma in human small intestine.
Expression
RNA: detected in 36 tissues Protein: detected in 47 cell types
RNA: detected in 36 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040933
Antibody HPA041299
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402146)
Target mass (kDa)
57.1
57.1
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA040933
Antibody HPA041299
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
