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Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm, the Golgi apparatus & actin filaments.
Antibody dilution
1:83
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023700
Antibody HPA053652
Standard validation
Approved
Figure description
Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
Antibody dilution
1:125
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023700
Antibody HPA053652
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421669)
Target mass (kDa)
105.2, 102, 101.6, 99
105.2, 102, 101.6, 99
Antibody dilution
1:250
1:420
PROTEIN ARRAY
Antibody HPA023700
Antibody HPA053652
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
