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Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp
Expression
RNA: detected in 30 tissues Protein: detected in 11 cell types
RNA: detected in 30 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH9
Antibody dilution
1:550
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044658
Antibody CAB002505
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
40.2, 40.1
40.2, 40.1, 12.5, 12.2
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA044658
Antibody CAB002505
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
