Fields »
ABOUT
HELP
BLOG
RMDN3

ANTIBODY INFORMATION
 
 

Antibody HPA009975

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA009975  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA009975

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA009975

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

 1:70  
 

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:69  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA009975

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA009975

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52.1, 34  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA009975

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA009975

  

Antigen

 Recombinant protein fragment  

Length (aa)

 133  

Antigen sequence

 
DEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADE
LHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEK
KSYALDGKEEAEAALEKGDESADCHLWYAVLCG
 

Matching transcripts

 RMDN3-001 - ENSP00000260385 [100%]
RMDN3-003 - ENSP00000342493 [100%]
RMDN3-012 - ENSP00000453535 [84%]
 

Other gene match

  
ANTIGEN VIEW
RMDN3-001
RMDN3-003
RMDN3-012

RELEVANT PUBLICATIONS
 
 

Antibody HPA009975

  

1.

 VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis
De Vos KJ et al
Hum Mol Genet 2012;21(6):1299-311
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.