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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Antibody dilution
1:64
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA036285
Antibody HPA059111
Standard validation
Approved
Figure description
Immunohistochemical staining of human adrenal gland shows strong membranous and cytoplasmic positivity in cortical cells.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
Antibody dilution
1:225
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036285
Antibody HPA059111
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412792)
Target mass (kDa)
93.7, 93.4, 81.9
93.7, 93.4, 81.9
Antibody dilution
1:250
1:210
PROTEIN ARRAY
Antibody HPA036285
Antibody HPA059111
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:2550
ANTIGEN INFORMATION
Antibody HPA036285
Antibody HPA059111
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
41
51
Antigen sequence
TFSVSLQKQNKMKFGKNMYINRDRIPEERNETVLKHSLEEE
LKEQMSAKRYYSALKTMEQLENVYFPWVSQYRFCQLMIENLPKLREDIKE
I
