FGF2 - Antibodies - The Human Protein Atlas

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ANTIBODY INFORMATION

Antibody CAB000125

Provider

Chemicon

Product name

AB1458

Host species

Rabbit

Clonality

pAb

Purity

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody CAB000125

Immunocytochemistry

Supported

Immunohistochemistry

Approved

Western blot

Uncertain

IMMUNOCYTOCHEMISTRY

Antibody CAB000125

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:2000

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody CAB000125

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in glial cells.

Expression

RNA: detected in 33 tissues
Protein: detected in 27 cell types

Retrieval

HIER pH9

Antibody dilution

1:3000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody CAB000125

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

30.8, 17.3

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody CAB000125

Antigen

Not known

Length (aa)

Antigen sequence

Matching transcripts