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FBN2

ANTIBODY INFORMATION
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA012853   3620.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   6  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA012853

  

Antibody CAB026401

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:25  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human placenta shows distinct positivity in basal membranes.   Immunohistochemical staining of human placenta shows distinct positivity in basal membranes.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 1 cell types		   RNA: detected in 11 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 314.8, 314.7, 157.7   314.8, 314.7, 157.7, 38.5, 36.8, 30  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA012853

  

Antibody CAB026401

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 130    

Antigen sequence

 
GMCFSGLVNGRCAQELPGRMTKMQCCCEPGRCWGIGTIPEACPVRGSEEY
RRLCMDGLPMGGIPGSAGSRPGGTGGNGFAPSGNGNGYGPGGTGFIPIPG
GNGFSPGVGGAGVGAGGQGPIITGLTILNQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					FBN2-001 - ENSP00000424571 [100%] 
FBN2-005 - ENSP00000425596 [100%] 
FBN2-201 - ENSP00000262464 [100%] 
FBN2-202 - ENSP00000482132 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FBN2-001
FBN2-005
FBN2-201
FBN2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA012853

												
										 
																				
											
											
											
Antibody CAB026401

												
										 
																		

																	

										
											
1.

												
																						MBTPS1/SKI-1/S1P proprotein convertase is required for ECM signaling and axial elongation during somitogenesis and vertebral development†

	Achilleos A et al

	Hum Mol Genet 2015;24(10):2884-98

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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