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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:59
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
WESTERN BLOT
Antibody HPA048739
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
55.1, 50.7
Antibody dilution
1:300
PROTEIN ARRAY
Antibody HPA048739
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:2350
ANTIGEN INFORMATION
Antibody HPA048739
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
CHVLKLPYQGNATMLVVLMEKMGDHLALEDYLTTDLVETWLRNMKTRNME
VFFPKFKLDQKYEMHELLRQMGIRRIFSPFADLSELSATGRNLQVSRVLQ
RTVIEVDERGTEAVAGILSEITAYSMPPVIKVDRPFHFMIYEETSGMLLF
L