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NTRK3

ANTIBODY INFORMATION
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA027484   HPA048065   sc-139  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 14   14   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry

    Supported  
Western blot

Uncertain   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to nucleoli & nuclear membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.    

Antibody dilution

 1:55   1:29    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.  

Expression

     RNA: detected in 29 tissues
Protein: detected in 47 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:80  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 97.1, 94.4, 93.5, 92.8, 91.8, 78.6, 68.5   97.1, 94.4, 93.5, 92.8, 91.8, 78.6, 69, 68.5   97.1, 94.4, 93.5, 92.8, 91.8, 78.6, 69, 68.5, 16.2, 10.7, 9.1  

Antibody dilution

 1:250   1:140   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1150    

ANTIGEN INFORMATION
 
 

Antibody HPA027484

  

Antibody HPA048065

  

Antibody CAB009233

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 86   93    

Antigen sequence

 
CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNASINITDISRNIT
SIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRS
  
DGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVITCNGSGSPLPDVD
WIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGFTLTCI
  

											 
																				

																		

										
											
Matching transcripts

												
																					NTRK3-001 - ENSP00000377990 [100%] 
NTRK3-002 - ENSP00000318328 [100%] 
NTRK3-004 - ENSP00000453959 [100%] 
NTRK3-015 - ENSP00000453511 [100%] 
NTRK3-018 - ENSP00000485864 [100%] 
NTRK3-019 - ENSP00000486784 [100%] 
NTRK3-201 - ENSP00000347397 [100%] 
NTRK3-202 - ENSP00000350356 [100%] 
NTRK3-203 - ENSP00000354207 [100%] 
NTRK3-204 - ENSP00000444673 [100%] 

											 
																						NTRK3-001 - ENSP00000377990 [100%] 
NTRK3-002 - ENSP00000318328 [100%] 
NTRK3-003 - ENSP00000437773 [100%] 
NTRK3-004 - ENSP00000453959 [100%] 
NTRK3-015 - ENSP00000453511 [100%] 
NTRK3-018 - ENSP00000485864 [100%] 
NTRK3-019 - ENSP00000486784 [100%] 
NTRK3-201 - ENSP00000347397 [100%] 
NTRK3-202 - ENSP00000350356 [100%] 
NTRK3-203 - ENSP00000354207 [100%] 
NTRK3-204 - ENSP00000444673 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

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NTRK3-001
NTRK3-002
NTRK3-003
NTRK3-004
NTRK3-015
NTRK3-018
NTRK3-019
NTRK3-201
NTRK3-202
NTRK3-203
NTRK3-204
								

							

						

							
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