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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells while islets and intercalated ducts were negative.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 27 cell types
RNA: detected in 36 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023245
Antibody HPA023285
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.1
57.1, 39.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA023245
Antibody HPA023285
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
