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Antibody staining overlaps with antibody HPA052412.
Validated
Antibody staining overlaps with antibody HPA044505.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubule organizing center.
Antibody dilution
1:47
1:81
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA044505
Antibody HPA052247
Antibody HPA052412
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Expression
RNA: detected in 30 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
Antibody dilution
1:120
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044505
Antibody HPA052247
Antibody HPA052412
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
97.3, 97.2
97.3, 97.2
97.3, 97.2, 54.9, 10.5
Antibody dilution
1:250
1:180
1:400
PROTEIN ARRAY
Antibody HPA044505
Antibody HPA052247
Antibody HPA052412
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
