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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:21
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Antibody dilution
1:21
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008228
Antibody HPA012021
Standard validation
Approved
Figure description
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in subset of hematopoietic cells.
Expression
RNA: detected in 36 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008228
Antibody HPA012021
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.4
61.4
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA008228
Antibody HPA012021
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
1:500
ANTIGEN INFORMATION
Antibody HPA008228
Antibody HPA012021
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
102
79
Antigen sequence
QPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAA
AAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYV
TV
