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NT5C3B

ANTIBODY INFORMATION
 
 

Antibody HPA030786

  

Antibody HPA057095

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030786   HPA057095  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA030786

  

Antibody HPA057095

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030786

  

Antibody HPA057095

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60   1:200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030786

  

Antibody HPA057095

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.4, 23.6   34.4, 25.8  

Antibody dilution

 1:250   1:340  

PROTEIN ARRAY
 
 

Antibody HPA030786

  

Antibody HPA057095

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:4150  

ANTIGEN INFORMATION
 
 

Antibody HPA030786

  

Antibody HPA057095

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   55  

Antigen sequence

 
GKRCPSSYNILDNSKIISEECRKELTALLHHYYPIEIDPHRTVKEKLPHM
VEWWTKAHNLLCQQKIQKFQIAQVVRESNAMLREGYKTFFNTLYHNNIPL
FIFSAGIGDILEEI
  
IVSNYMDFNEDGFLQGFKGQLIHTYNKNSSVCENCGYFQQLEGKTNVILL
GDSIG
 

Matching transcripts

 NT5C3B-008 - ENSP00000389948 [100%]
NT5C3B-017 - ENSP00000429878 [100%]
  NT5C3B-008 - ENSP00000389948 [96%]
NT5C3B-009 - ENSP00000397742 [96%]
 

Other gene match

    
ANTIGEN VIEW
NT5C3B-008
NT5C3B-009
NT5C3B-017
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.