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COL6A2

ANTIBODY INFORMATION
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007029   HPA030920   HPA038799  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 3   16   16  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  
Immunocytochemistry

Uncertain      
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Standard validation

 Uncertain      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.      

Antibody dilution

 1:10      

Literature conformity

 The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human vagina shows strong positivity in extracellular matrix and blood vessels.   Immunohistochemical staining of human duodenum shows extracellular matrix positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 18 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:500   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 108.6, 97.4, 87.3, 20   108.6, 97.4, 87.3   108.6  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:12000  

ANTIGEN INFORMATION
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 120   101   76  

Antigen sequence

 
TTERNNNCPEKTDCPIHVYFVLDTSESVTMQSPTDILLFHMKQFVPQFIS
QLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFIKNLQGISSFR
RGTFTDCALANMTEQIRQDR
  
KGTVHFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVAPNQNLKEQG
LRDIASTPHELYRNDYATMLPDSTEIDQDTINRIIKVMKHEAYGECYKVS
C
  
ELSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSL
GDRAAVFHEKDYDSLAQPGFFDRFIR
 

Matching transcripts

 COL6A2-001 - ENSP00000300527 [100%]
COL6A2-002 - ENSP00000380870 [100%]
COL6A2-003 - ENSP00000387115 [100%]
COL6A2-004 - ENSP00000390418 [100%]
COL6A2-201 - ENSP00000312529 [100%]
  COL6A2-001 - ENSP00000300527 [100%]
COL6A2-002 - ENSP00000380870 [100%]
COL6A2-003 - ENSP00000387115 [100%]
COL6A2-201 - ENSP00000312529 [100%]
  COL6A2-001 - ENSP00000300527 [100%]
 

Other gene match

      
ANTIGEN VIEW
COL6A2-001
COL6A2-002
COL6A2-003
COL6A2-004
COL6A2-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA007029

  

Antibody HPA030920

  

Antibody HPA038799

  

1.

 Vascular gene expression patterns are conserved in primary and metastatic brain tumors
Liu Y et al
J Neurooncol 2010;99(1):13-24
      

2.

 Dissecting the oncogenic and tumorigenic potential of differentiated human induced pluripotent stem cells and human embryonic stem cells
Ghosh Z et al
Cancer Res 2011;71(14):5030-9
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.