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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments.
Antibody dilution
1:50
1:25
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027093
Antibody CAB025807
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside reaction centra.
Expression
RNA: detected in 34 tissues Protein: detected in 20 cell types
RNA: detected in 34 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027093
Antibody CAB025807
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409681)