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TOR1AIP1

ANTIBODY INFORMATION
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047151   HPA050546  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA047151

  

Antibody HPA050546

  
Immunocytochemistry

 

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by one siRNA.		  

 
 

Relative fluorescence intensity (RFI)

  

Figure description

   Nuclear region of segmented cells in 10x-images  

Antibody dilution

   1:172  
 

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nuclear membrane.  

Antibody dilution

   1:172  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA050546 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA047151 across 45 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nuclear membrane positivity.   Immunohistochemical staining of human cerebellum shows strong nuclear membrane positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:600  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.8, 66.3, 66.2, 22.4   67.8, 66.3, 66.2, 52.4, 22.4, 22.2  

Antibody dilution

 1:1920   1:860  

PROTEIN ARRAY
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15350   1:6900  

ANTIGEN INFORMATION
 
 

Antibody HPA047151

  

Antibody HPA050546

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   99  

Antigen sequence

 
EEFRSDSAKEEVRESAYYLRSRQRRQPRPQETEEMKTRRTTRLQQQHSEQ
PPLQPSPVTTRRGLRDSHSSEEDEASSQTDLSQTISKKTVRS
  
EATSVQQKVNFSEEGETEEDDQDSSHSSVTTVKARSRDSDESGDKTTRSS
SQYIESFWQSSQSQNFTAHDKQRSVLSSGYQKTPQEWAPQTARIRTRMQ
 

Matching transcripts

 TOR1AIP1-001 - ENSP00000435365 [99%]
TOR1AIP1-004 - ENSP00000476687 [99%]
TOR1AIP1-011 - ENSP00000271583 [99%]
TOR1AIP1-008 - ENSP00000434316 [88%]
  TOR1AIP1-001 - ENSP00000435365 [99%]
TOR1AIP1-004 - ENSP00000476687 [99%]
TOR1AIP1-011 - ENSP00000271583 [99%]
TOR1AIP1-012 - ENSP00000432717 [99%]
TOR1AIP1-015 - ENSP00000393292 [99%]
TOR1AIP1-008 - ENSP00000434316 [85%]
 

Other gene match

    
ANTIGEN VIEW
TOR1AIP1-001
TOR1AIP1-004
TOR1AIP1-008
TOR1AIP1-011
TOR1AIP1-012
TOR1AIP1-015
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.