POGZ - Antibodies - The Human Protein Atlas

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POGZ

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA006800

Antibody HPA008781

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA006800

HPA008781

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA006800

Antibody HPA008781

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006800

Antibody HPA008781

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:55

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA008781.

Validated

Antibody staining overlaps with antibody HPA006800.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Antibody dilution

1:56

1:30

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006800

Antibody HPA008781

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.

Immunohistochemical staining of human bronchus shows strong membranous and nuclear positivity in respiratory epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 76 cell types

RNA: detected in 37 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:50

Literature conformity

Consistent with gene/protein characterization data.

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006800

Antibody HPA008781

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

155.3, 154.5, 151.2, 150.1, 149.3, 145.3

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA006800

Antibody HPA008781

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA006800

Antibody HPA008781

Antigen

Recombinant protein fragment

Length (aa)

125

139

Antigen sequence

GEPWCDVVLAILADGTVLPTLVFYRGQMDQPANMPDSILLEAKESGYSDD
EIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAV
VPAGCSSKIQPLDVCIKRTVKNFLH

PEELLTPLAPALPSPASTATPPPTPTHPQALALPPLATEGAECLNVDDQD
EGSPVTQEPELASGGGGSGGVGKKEQLSVKKLRVVLFALCCNTEQAAEHF
RNPQRRIRRWLRRFQASQGENLEGKYLSFEAEEKLAEWV

Matching transcripts

POGZ-001 - ENSP00000271715 [100%]
POGZ-002 - ENSP00000376484 [100%]
POGZ-003 - ENSP00000357856 [100%]
POGZ-011 - ENSP00000418408 [100%]
POGZ-016 - ENSP00000386836 [100%]
POGZ-017 - ENSP00000431259 [100%]

Other gene match

ANTIGEN VIEW

POGZ-001	POGZ-002	POGZ-003	POGZ-011	POGZ-016	POGZ-017

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.