Fields »
ABOUT
HELP
BLOG
S100A8

ANTIBODY INFORMATION
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA024372   CBL269  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 5   1  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA024372

  

Antibody CAB002791

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line HaCaT shows localization to intermediate filaments.    

Antibody dilution

 1:68    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in a subset of cells outside reaction centra .   Immunohistochemical staining of human esophagus shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 14 cell types		   RNA: detected in 36 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:5000   1:1  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 10.8   10.8  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024372

  

Antibody CAB002791

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 77    

Antigen sequence

 
LTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGA
DVWFKELDINTDGAVNFQEFLILVIKM
   

Matching transcripts

 S100A8-001 - ENSP00000357722 [100%]
S100A8-002 - ENSP00000357721 [100%]
   

Other gene match

    
ANTIGEN VIEW
S100A8-001
S100A8-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA024372

  

Antibody CAB002791

  

1.

 Comparative proteomic analysis reveals activation of mucosal innate immune signaling pathways during cholera
Ellis CN et al
Infect Immun 2015;83(3):1089-103
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.