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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human oral mucosa shows distinct cytoplasmic, membranous and nuclear positivity in squamous epithelial cells.
Expression
RNA: detected in 0 tissues Protein: detected in 51 cell types
RNA: detected in 0 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:450
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042223
Antibody HPA042772
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408486)
Target mass (kDa)
52.3
52.3
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA042223
Antibody HPA042772
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
