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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:54
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA015473
Antibody HPA056866
Standard validation
Approved
Figure description
Immunohistochemical staining of human duodenum shows cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:80
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015473
Antibody HPA056866
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408287)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.1
37.1, 28, 27.4
Antibody dilution
1:250
1:180
PROTEIN ARRAY
Antibody HPA015473
Antibody HPA056866
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:2150
ANTIGEN INFORMATION
Antibody HPA015473
Antibody HPA056866
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
29
52
Antigen sequence
ELEEGIDVLDSDGNLVGSSKIAARHALLE
RLREAVQLLEDYKHGTLRPGVTNEQLWSAQKIKQAILHPDTNEKIFMPFR
MS
