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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, cell junctions & vesicles.
Antibody dilution
1:26
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035666
Antibody HPA045884
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong positivity in nerve fibers.
Expression
RNA: detected in 2 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035666
Antibody HPA045884
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406033)
Target mass (kDa)
27.3, 20.5, 18.5, 13.5
27.3, 20.5, 18.5, 13.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA035666
Antibody HPA045884
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
