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Antibody staining overlaps with antibody HPA053192.
Validated
Antibody staining overlaps with antibody HPA036837.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Antibody dilution
1:98
1:154
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA036837
Antibody HPA036838
Antibody HPA053192
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 43 cell types
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036837
Antibody HPA036838
Antibody HPA053192
Standard validation
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403266)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
121.4, 120.3
121.4, 120.3
121.4, 120.3, 10.9
Antibody dilution
1:250
1:250
1:770
PROTEIN ARRAY
Antibody HPA036837
Antibody HPA036838
Antibody HPA053192
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
