BOC - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

BOC

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA060778

HPA061787

MAB20361

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA061787.

Validated

Antibody staining overlaps with antibody HPA060778.

Standard validation

Approved

Supported

Figure description

Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & plasma membrane.

Antibody dilution

1:45

1:4

1:37

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 80 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

121.2, 121.1

121.2, 121.1, 16.3, 9, 7.3, 4.1, 3.5, 2.5

Antibody dilution

1:150

1:80

1:500

PROTEIN ARRAY

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1800

1:500

ANTIGEN INFORMATION

Antibody HPA060778

Antibody HPA061787

Antibody CAB025806

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

KHTTDLGFPRSALPPSCPYTMVPLGGLPGHQASGQPYLSGISGRACANGI
HMNRGCPSAAVGYPGMKPQQHCPGELQQQSDTSSLL

PSITPRLWQDAELATGTPPVSPSKLGNPEQMLRGQPALPRPPTSVGPASP
QCPGEKGQGAPAEAPIILSS

Matching transcripts

BOC-001 - ENSP00000418663 [100%]
BOC-003 - ENSP00000273395 [100%]
BOC-201 - ENSP00000347546 [100%]

Other gene match

ANTIGEN VIEW

BOC-001	BOC-003	BOC-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.