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SLIT2

ANTIBODY INFORMATION
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA019511   HPA023088   AB5701  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   5   3  

References

   3    

VALIDATION SUMMARY
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 52 cell types		   RNA: detected in 35 tissues
Protein: detected in 70 cell types		   RNA: detected in 35 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:35   1:850  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 171.3, 169.9, 169.4, 168.9, 160, 8.5   171.3, 169.9, 169.4, 168.9, 160   171.3, 169.9, 169.4, 168.9, 160, 23.1, 21.7, 16.7, 8.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 84   89    

Antigen sequence

 
SAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFD
SPLYVGGMPGKSNVASLRQAPGQNGTSFHGRIWN
  
LYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQE
GWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLE
   

Matching transcripts

 SLIT2-001 - ENSP00000422591 [98%]
SLIT2-002 - ENSP00000427548 [98%]
SLIT2-003 - ENSP00000422261 [98%]
SLIT2-004 - ENSP00000273739 [98%]
SLIT2-201 - ENSP00000482129 [98%]
SLIT2-011 - ENSP00000423179 [82%]
  SLIT2-001 - ENSP00000422591 [100%]
SLIT2-002 - ENSP00000427548 [100%]
SLIT2-003 - ENSP00000422261 [100%]
SLIT2-004 - ENSP00000273739 [100%]
SLIT2-201 - ENSP00000482129 [100%]
   

Other gene match

      
ANTIGEN VIEW
SLIT2-001
SLIT2-002
SLIT2-003
SLIT2-004
SLIT2-011
SLIT2-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA019511

  

Antibody HPA023088

  

Antibody CAB007590

  

1.

   The neuronal repellent SLIT2 is a target for repression by EZH2 in prostate cancer
Yu J et al
Oncogene 2010;29(39):5370-80
    

2.

   Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium
Brantley-Sieders DM et al
Cancer Res 2011;71(3):976-87
    

3.

   Loss of expression and promoter methylation of SLIT2 are associated with sessile serrated adenoma formation
Beggs AD et al
PLoS Genet 2013;9(5):e1003488
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.