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EIF2B5

ANTIBODY INFORMATION
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA064370   HPA069303   sc-55558  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 14   14   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

    Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA069303.		  
Validated

Antibody staining overlaps with antibody HPA064370.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.   Immunofluorescent staining of human cell line RT4 shows localization to cytosol.    

Antibody dilution

 1:185   1:36    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Standard validation

     Uncertain  

     

Figure description

     Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:75  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 80.4, 78.3   80.4, 78.3   80.4, 78.3, 11.2  

Antibody dilution

 1:411   1:120   1:500  

PROTEIN ARRAY
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:7400   1:1300    

ANTIGEN INFORMATION
 
 

Antibody HPA064370

  

Antibody HPA069303

  

Antibody CAB015412

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 102   76    

Antigen sequence

 
SILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVA
AGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHP
PD
  
MDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVL
SHVVLEFPLQQMDSPLDSSRYCALLL
  

											 
																				

																		

										
											
Matching transcripts

												
																					EIF2B5-001 - ENSP00000273783 [100%] 
EIF2B5-017 - ENSP00000409142 [100%] 

											 
																						EIF2B5-001 - ENSP00000273783 [100%] 
EIF2B5-017 - ENSP00000409142 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EIF2B5-001
EIF2B5-017
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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