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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA001525 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA001524 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human blood vessels shows distinct positivity in plasma.
Immunohistochemical staining of human kidney shows extra-cellular positivity in blood vessels and cytoplasmic positivity in cells of renal tubules.
Immunohistochemical staining of human blood vessel shows strong positivity of blood plasma.
Expression
RNA: detected in 3 tissues Protein: detected in 15 cell types
RNA: detected in 3 tissues Protein: detected in 7 cell types
RNA: detected in 3 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:150
1:600
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001524
Antibody HPA001525
Antibody CAB026209
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400610)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400610)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.4, 39.3
39.4, 39.3
39.4, 39.3
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001524
Antibody HPA001525
Antibody CAB026209
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
