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DGKQ

ANTIBODY INFORMATION
 
 

Antibody HPA026797

  

Antibody CAB033835

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA026797   sc-66861  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 6   12  

References

 3    

VALIDATION SUMMARY
 
 

Antibody HPA026797

  

Antibody CAB033835

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026797

  

Antibody CAB033835

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in superficial glandular cells.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells .  

Expression

 RNA: detected in 37 tissues
Protein: detected in 28 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:60  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026797

  

Antibody CAB033835

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 101.2, 94.8   101.2, 94.8, 17, 16.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA026797

  

Antibody CAB033835

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA026797

  

Antibody CAB033835

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 101    

Antigen sequence

 
HVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVA
CFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKS
G
  

											 
																				

																		

										
											
Matching transcripts

												
																					DGKQ-001 - ENSP00000273814 [100%] 
DGKQ-002 - ENSP00000425862 [87%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DGKQ-001
DGKQ-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA026797

												
										 
																				
											
											
											
Antibody CAB033835

												
										 
																		

																	

										
											
1.

												
																						Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells

	Cai K et al

	Biochim Biophys Acta 2014;1841(4):552-62

			
											 
																						
											 
																				

																		

										
											
2.

												
																						cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1

	Cai K et al

	J Lipid Res 2013;54(8):2121-32

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Diacylglycerol kinase θ couples farnesoid X receptor-dependent bile acid signalling to Akt activation and glucose homoeostasis in hepatocytes

	Cai K et al

	Biochem J 2013;454(2):267-74

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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