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C7orf43

ANTIBODY INFORMATION
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019359   HPA029463   HPA029464  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   6   6  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA029463.		  
Validated

Antibody staining overlaps with antibody HPA019359.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to microtubule organizing center & vesicles.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, microtubule organizing center & vesicles.    

Antibody dilution

 1:100   1:100    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:50   1:50  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62.6, 34.4   62.6   62.6, 34.4  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:30000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 119   112   116  

Antigen sequence

 
LRTGLFELSQHMKLKLQFTASVSHPPPEARPLSRKSSPSSPAVRDLVERH
QASLGRSQSFSHQQPSRSHLMRSGSVMERRAITPPVASPVGRPLYLPPDK
AVLSLDKIAKRECKVLVVE
  
ETFRGEQSAFKAQVSTLLTLLPPPVLRCRQFTVAGKHLTVLKVLNSSSQE
EISIWDIRILPNFNASYLPVMPDGSVLLVDNVCHQSGEVSMGSFCRLPGT
SGCFPCPLNALE
  
LEVPLIAVVQWSTPKLPFTQSIYTHYRLPSVRLDRPCFVMTASCKSPVRT
YERFTVTYTLLNNLQDFLAVRLVWTPEHAQAGKQLCEEERRAMQAALDSV
VCHTPLNNLGFSRKGS
 

Matching transcripts

 C7orf43-001 - ENSP00000324741 [100%]
C7orf43-008 - ENSP00000389672 [100%]
C7orf43-010 - ENSP00000396432 [100%]
  C7orf43-001 - ENSP00000324741 [100%]
  C7orf43-001 - ENSP00000324741 [100%]
C7orf43-008 - ENSP00000389672 [100%]
C7orf43-010 - ENSP00000396432 [100%]
 

Other gene match

      
ANTIGEN VIEW
C7orf43-001
C7orf43-008
C7orf43-010

RELEVANT PUBLICATIONS
 
 

Antibody HPA019359

  

Antibody HPA029463

  

Antibody HPA029464

  

1.

   Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.