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TRIM4

ANTIBODY INFORMATION
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019356   HPA029461  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA019356

  

Antibody HPA029461

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.    

Antibody dilution

 1:102    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic and nuclear positivity in bone marrow poietic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:275  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.5, 54.2, 34.2, 23.4   57.5, 54.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019356

  

Antibody HPA029461

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 108   105  

Antigen sequence

 
KSQRNLVAKMKKVMHLQDVEVKNATQWKDKIKSQRMRISTEFSKLHNFLV
EEEDLFLQRLNKEEEETKKKLNENTLKLNQTIASLKKLILEVGEKSQAPT
LELLQNPK
  
VAVGVCREDVMGITDRSKMSPDVGIWAIYWSAAGYWPLIGFPGTPTQQEP
ALHRVGVYLDRGTGNVSFYSAVDGVHLHTFSCSSVSRLRPFFWLSPLASL
VIPPV
 

Matching transcripts

 TRIM4-001 - ENSP00000275736 [100%]
TRIM4-002 - ENSP00000396229 [100%]
TRIM4-004 - ENSP00000348216 [100%]
TRIM4-005 - ENSP00000346186 [100%]
  TRIM4-001 - ENSP00000275736 [100%]
TRIM4-004 - ENSP00000348216 [100%]
 

Other gene match

    
ANTIGEN VIEW
TRIM4-001
TRIM4-002
TRIM4-004
TRIM4-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.