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AGBL3

ANTIBODY INFORMATION
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020397   HPA024150  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA020397

  

Antibody HPA024150

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:50   1:100  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 28 tissues
Protein: detected in 47 cell types		   RNA: detected in 28 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:250  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 107, 82.1   107, 82.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020397

  

Antibody HPA024150

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   90  

Antigen sequence

 
CPEPVYIPTGLETQPLYPDSKEATVVYLAEDAYKEPCFVYSRVGGNRTPL
KQPVDYRDNTLMFEARFESGNLQKVVKVAEYEY
  
DYSDRTISDEDESDEDMFMKFVSEDLHRCALLTADSFGDPFFPRTTQILL
EYQLGRWVPRLREPRDLYGVSSSGPLSPTRWPYHCEVIDE
 

Matching transcripts

 AGBL3-006 - ENSP00000401220 [99%]
AGBL3-007 - ENSP00000388275 [99%]
  AGBL3-006 - ENSP00000401220 [100%]
AGBL3-007 - ENSP00000388275 [100%]
 

Other gene match

    
ANTIGEN VIEW
AGBL3-006
AGBL3-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.