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Antibody staining overlaps with antibody HPA058586.
Validated
Antibody staining overlaps with antibody HPA055855.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.
Antibody dilution
1:98
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA055855
Antibody HPA058586
Antibody CAB070418
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Expression
RNA: detected in 35 tissues Protein: detected in 28 cell types
RNA: detected in 35 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:85
1:375
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055855
Antibody HPA058586
Antibody CAB070418
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
47.3
49.9, 47.3, 46, 44.5, 43.7, 41.1
49.9, 47.3, 46, 44.5, 43.7, 41.1, 13
Antibody dilution
1:490
1:170
1:500
PROTEIN ARRAY
Antibody HPA055855
Antibody HPA058586
Antibody CAB070418
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
