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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
Antibody dilution
1:10
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA024663
Antibody HPA026580
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows moderate cytopolasmic positivity in tubular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity combined with distinct granular cytoplasmic staining in glandular cells.
Expression
RNA: detected in 29 tissues Protein: detected in 69 cell types
RNA: detected in 29 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:500
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA024663
Antibody HPA026580
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403156)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403156)
Target mass (kDa)
26, 22.7, 11.8
26, 22.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA024663
Antibody HPA026580
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
