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Antibody staining overlaps with antibody HPA014979.
Validated
Antibody staining overlaps with antibody HPA014760.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line AF22 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line AF22 shows localization to nucleoplasm.
Antibody dilution
1:5
1:7
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA014760
Antibody HPA014968
Antibody HPA014979
Standard validation
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
Expression
RNA: detected in 5 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014760
Antibody HPA014968
Antibody HPA014979
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413579)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.8
35.8
35.8
Antibody dilution
1:250
1:125
1:250
PROTEIN ARRAY
Antibody HPA014760
Antibody HPA014968
Antibody HPA014979
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
