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ADAM12

ANTIBODY INFORMATION
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030866   HPA030867   HPA030868  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   6   12  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.    

Antibody dilution

   1:200    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in torphoblastic cells.   Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 16 tissues
Protein: detected in 9 cell types		   RNA: detected in 16 tissues
Protein: detected in 26 cell types		   RNA: detected in 16 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:90   1:8000   1:500  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 99.5, 80.4, 31.1   99.5   99.5, 80.4  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:600000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 67   69   90  

Antigen sequence

 
RGVSLWNQGRADEVVSASVRSGDLWIPVKSFDSKNHPEVLNIRLQRESKE
LIINLERNEGLIASSFT
  
LLFTNKKTTIEKLRCVRPSRPPRGFQPCQAHLGHLGKGLMRKPPDSYPPK
DNPRRLLQCQNVDISRPLN
  
VTLAHELGHNFGMNHDTLDRGCSCQMAVEKGGCIMNASTGYPFPMVFSSC
SRKDLETSLEKGMGVCLFNLPEVRESFGGQKCGNRFVEEG
 

Matching transcripts

 ADAM12-001 - ENSP00000357668 [99%]
ADAM12-002 - ENSP00000357665 [99%]
ADAM12-003 - ENSP00000391268 [99%]
  ADAM12-001 - ENSP00000357668 [100%]
  ADAM12-001 - ENSP00000357668 [100%]
ADAM12-002 - ENSP00000357665 [100%]
 

Other gene match

      
ANTIGEN VIEW
ADAM12-001
ADAM12-002
ADAM12-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA030866

  

Antibody HPA030867

  

Antibody HPA030868

  

1.

   Profiling post-centrifugation delay of serum and plasma with antibody bead arrays
Qundos U et al
J Proteomics 2013;95:46-54
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.