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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.
Antibody dilution
1:54
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA052700
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human soft tissue shows cytoplasmic positivity in peripheral nerve.
Expression
RNA: detected in 14 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:600
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052700
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
44.1, 43.8
Antibody dilution
1:270
PROTEIN ARRAY
Antibody HPA052700
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
