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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & midbody.
Antibody dilution
1:182
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA070550
Antibody CAB013292
Standard validation
Supported
Figure description
Immunohistochemical staining of human tonsil shows nuclear positivity in reaction center cells.
Expression
RNA: detected in 35 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA070550
Antibody CAB013292
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
105.4, 105
105.4, 105, 19
Antibody dilution
1:610
1:500
PROTEIN ARRAY
Antibody HPA070550
Antibody CAB013292
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
