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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA061503 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA037593 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA037594 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA037593 across 72 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia.
Expression
RNA: detected in 9 tissues Protein: detected in 4 cell types
RNA: detected in 9 tissues Protein: detected in 2 cell types
RNA: detected in 9 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:1300
1:150
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037593
Antibody HPA037594
Antibody HPA061503
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408146)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408146)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408146)
Target mass (kDa)
29.5, 29.4, 22.4, 14.4
29.5, 29.4, 22.4
29.5, 29.4
Antibody dilution
1:250
1:250
1:125
PROTEIN ARRAY
Antibody HPA037593
Antibody HPA037594
Antibody HPA061503
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
