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BAG3

ANTIBODY INFORMATION
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA018493   HPA020586  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA018493

  

Antibody HPA020586

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated

Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA020586.		  
Validated

Antibody staining overlaps with antibody HPA018493.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:50   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA020586 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA018493 across 45 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human oral mucosa shows strong positivity in squamous epithelial cells.   Immunohistochemical staining of human prostate shows distinct positivity in basal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:100  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 61.6   61.6, 34.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA018493

  

Antibody HPA020586

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 110   134  

Antigen sequence

 
DSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNL
EADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSM
TDTPGNPAAP
  
DCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQ
AQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSRE
GSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRET
 

Matching transcripts

 BAG3-001 - ENSP00000358081 [100%]
  BAG3-001 - ENSP00000358081 [100%]
BAG3-002 - ENSP00000410036 [99%]
 

Other gene match

    
ANTIGEN VIEW
BAG3-001
BAG3-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.