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SPC25

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GENERAL INFORMATION

Gene name

 SPC25

Gene description

 SPC25, NDC80 kinetochore complex component

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 2
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the cytosol.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Cytosol (validated)

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
HPA047144 , HPA050544
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ASSAYS
RNA expression
Human cells
siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the cytosol.

Main location

 Cytosol (validated)

 

 

 
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Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA047144 U-2 OS Cytosol
HPA047144 U-251 MG Cytosol
HPA050544 U-2 OS Cytosol
HPA050544 U-251 MG Cytosol

siRNA VALIDATION
 

Relative fluorescence intensity (RFI)

Antibody

 HPA047144

siRNA validation

 Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

 Whole cells of segmented cells in 10x-images

siRNA 2

RFI

 55%

p-value

 < 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

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Thumbnail 40x

Antibody

Sample

Cell line
HPA047144 siRNA 2 U-2 OS
HPA047144 scrambled U-2 OS

GENE INFORMATION

Gene name

 SPC25 (HGNC Symbol)

Synonyms

 AD024, MGC22228, SPBC25

Description

 SPC25, NDC80 kinetochore complex component (HGNC Symbol)

Entrez gene summary

 This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008]

Chromosome

 2

Cytoband

Chromosome location (bp)

 168834132 - 168913371

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000152253 (version 83.38)

Entrez gene

 57405

UniProt

 Q9HBM1 (UniProt - Evidence at protein level)

neXtProt

 NX_Q9HBM1

Antibodypedia

 SPC25 antibodies

PROTEIN BROWSER
SPC25-001
SPC25-004

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
SPC25-001
ENSP00000282074
ENST00000282074
Q9HBM1 [Direct mapping]
Kinetochore protein Spc25
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000278 [mitotic cell cycle]
GO:0000777 [condensed chromosome kinetochore]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005829 [cytosol]
GO:0007052 [mitotic spindle organization]
GO:0007059 [chromosome segregation]
GO:0007067 [mitotic nuclear division]
GO:0007264 [small GTPase mediated signal transduction]
GO:0031262 [Ndc80 complex]
GO:0051301 [cell division]
Show all
 224 aa
26.2 kDa 		No 0
SPC25-004
ENSP00000393322
ENST00000451987
C9JW94 [Direct mapping]
Kinetochore protein Spc25
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 115 aa
13.6 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.