NADK2 - Antibodies - The Human Protein Atlas

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NADK2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038366

HPA038367

HPA061492

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Immunohistochemistry

Western blot

Protein array

IMMUNOHISTOCHEMISTRY

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA061492 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA038367 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 77 cell types

RNA: detected in 37 tissues
Protein: detected in 63 cell types

RNA: detected in 37 tissues
Protein: detected in 55 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:1200

1:800

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.4, 46, 34.2, 31.7, 28.3, 15.3

49.4, 34.2, 31.7

49.4, 46, 34.2, 31.7, 28.3, 9.6

Antibody dilution

1:250

1:360

PROTEIN ARRAY

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

1:4300

ANTIGEN INFORMATION

Antibody HPA038366

Antibody HPA038367

Antibody HPA061492

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

RELVEKVTNEYNESLLYSPEEPKILFSIREPIANRVFSSSRQRCFSSKVC
VRSRCWDACMVVDGGTSFEFNDGAIASMMINKEDELRTVLLE

RALNIERAHDERSEASGPQLLPVRALNEVFIGESLSSRASYYEISVDDGP
WEKQKSSGLNLCTGTGSKAWSFNINRVATQAVEDVLNIAKRQGNLSL

RLKPVIGVNTDPERSEGHLCLPVRYTHSFPEALQKFYRGEFRWLWRQRIR
LYLEGTGINPVPVDLHEQQLSLNQHN

Matching transcripts

NADK2-001 - ENSP00000380499 [100%]
NADK2-002 - ENSP00000422250 [100%]
NADK2-003 - ENSP00000371362 [100%]
NADK2-004 - ENSP00000421029 [100%]
NADK2-010 - ENSP00000282512 [100%]
NADK2-201 - ENSP00000480506 [100%]
NADK2-009 - ENSP00000424166 [93%]

NADK2-001 - ENSP00000380499 [100%]
NADK2-003 - ENSP00000371362 [100%]
NADK2-010 - ENSP00000282512 [100%]
NADK2-002 - ENSP00000422250 [82%]

Other gene match

ANTIGEN VIEW

NADK2-001	NADK2-002	NADK2-003	NADK2-004	NADK2-007	NADK2-009	NADK2-010	NADK2-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.