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DDX4

ANTIBODY INFORMATION
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA037763   HPA037764   MAB2030  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 10   10   10  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and moderate staining in Leydig cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong positivity in developing sperm cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 10 cell types		   RNA: detected in 1 tissues
Protein: detected in 5 cell types		   RNA: detected in 1 tissues
Protein: detected in 37 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:600   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 79.3, 77.1, 75.8, 63.2   79.3, 77.1, 75.8, 63.2, 56.5   79.3, 77.1, 75.8, 63.2, 56.5, 17.5, 17.3, 15.5, 11.6  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA037763

  

Antibody HPA037764

  

Antibody CAB026170

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 89   78    

Antigen sequence

 
NTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSG
STRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD
  
GGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHD
APPAILTFEEANLCQTLNNNIAKAGYTK
  

											 
																				

																		

										
											
Matching transcripts

												
																					DDX4-001 - ENSP00000424838 [100%] 
DDX4-003 - ENSP00000347087 [100%] 
DDX4-005 - ENSP00000423123 [100%] 
DDX4-201 - ENSP00000334167 [100%] 
DDX4-202 - ENSP00000425359 [100%] 

											 
																						DDX4-001 - ENSP00000424838 [100%] 
DDX4-002 - ENSP00000427167 [100%] 
DDX4-003 - ENSP00000347087 [100%] 
DDX4-005 - ENSP00000423123 [100%] 
DDX4-201 - ENSP00000334167 [100%] 
DDX4-202 - ENSP00000425359 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
DDX4-001
DDX4-002
DDX4-003
DDX4-005
DDX4-201
DDX4-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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