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Antibody staining overlaps with antibody HPA057328.
Validated
Antibody staining overlaps with antibody HPA055032.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Antibody dilution
1:123
1:75
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA055032
Antibody HPA057328
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human hippocampus shows distinct positivity in neuropil.
Expression
RNA: detected in 37 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055032
Antibody HPA057328
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
55.8, 51.1, 48.6, 47.6, 42.5
55.8, 51.1, 48.6, 47.6, 42.5
Antibody dilution
1:620
1:250
PROTEIN ARRAY
Antibody HPA055032
Antibody HPA057328
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
