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Immunofluorescent staining of human cell line HeLa shows localization to cytosol.
Antibody dilution
1:24
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003160
Antibody HPA048156
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
Expression
RNA: detected in 9 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003160
Antibody HPA048156
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26, 25, 14.1
26
Antibody dilution
1:250
1:120
PROTEIN ARRAY
Antibody HPA003160
Antibody HPA048156
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
