Fields »
ABOUT
HELP
BLOG
DDAH1

ANTIBODY INFORMATION
 
 

Antibody HPA006308

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006308  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

 1  

VALIDATION SUMMARY
 
 

Antibody HPA006308

  
Immunocytochemistry

Approved  
Immunohistochemistry


Validated
  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006308

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:65  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006308

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human kidney shows strong nuclear and cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:600  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006308

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.1, 20.5, 20.2  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA006308

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA006308

  

Antigen

 Recombinant protein fragment  

Length (aa)

 131  

Antigen sequence

 
SKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSE
SAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEY
PESAKVYEKLKDHMLIPVSMSELEKVDGLLT
 

Matching transcripts

 DDAH1-001 - ENSP00000284031 [100%]
DDAH1-009 - ENSP00000439045 [100%]
DDAH1-010 - ENSP00000488725 [100%]
DDAH1-201 - ENSP00000411189 [100%]
DDAH1-202 - ENSP00000438604 [100%]
 

Other gene match

  
ANTIGEN VIEW
DDAH1-001
DDAH1-009
DDAH1-010
DDAH1-201
DDAH1-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA006308

  

1.

 Pulmonary hypertension associated with advanced systolic heart failure: dysregulated arginine metabolism and importance of compensatory dimethylarginine dimethylaminohydrolase-1
Shao Z et al
J Am Coll Cardiol 2012;59(13):1150-8
Application: IHC 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.