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CCSAP

ANTIBODY INFORMATION
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA028402   HPA043443  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA028402

  

Antibody HPA043443

  
Immunocytochemistry

Uncertain   Uncertain  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytokinetic bridge.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.  

Antibody dilution

 1:50   1:88  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neurons.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 20 cell types		   RNA: detected in 35 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:135   1:600  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.2, 17.8   30.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA028402

  

Antibody HPA043443

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   88  

Antigen sequence

 
KQTDTGSQKTHNVCASAPVHEIHESALRAKNRRQVEKRKLVAQRQRAHSV
DVEKNRKMKASSSENPWMTEYMRCYSARA
  
EEQDAEAGDAEAEDAEDAALPALPVKDVEDKPEQQTRTRETDKSPTSTEP
RQQPSALFARGNRKAVKSPQRSSSKIKENKHPFALYGW
 

Matching transcripts

 CCSAP-001 - ENSP00000355648 [100%]
CCSAP-003 - ENSP00000355647 [100%]
CCSAP-201 - ENSP00000284617 [100%]
  CCSAP-001 - ENSP00000355648 [100%]
CCSAP-201 - ENSP00000284617 [100%]
 

Other gene match

    
ANTIGEN VIEW
CCSAP-001
CCSAP-003
CCSAP-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.