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CXADR

ANTIBODY INFORMATION
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA003342   HPA030411   HPA030412   sc-15405  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 AC136352.5 - ENSG00000277666 [80%]
        

Released in version

 3   6   6   2  

References

   3      

VALIDATION SUMMARY
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  
Immunocytochemistry

  Supported     Supported  
Immunohistochemistry

Supported  

Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Approved   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Standard validation

   Supported     Supported  

       

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cell junctions.     Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.  

Antibody dilution

   1:100     1:13  

Literature conformity

   The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

 

  
 

 

  
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent

  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
 

  
 

    

Figure description

   Distribution of protein expression (antibody staining).
Pearson correlation with HPA030412 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA030411 across 46 cell lines.		    
 

Formal validation: Independent

  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
 

  
 

    

Figure description

   Distribution of protein expression (antibody staining).
Spearman correlation with HPA030412 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA030411 across 73 cell types.		    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 28 cell types		   RNA: detected in 32 tissues
Protein: detected in 61 cell types		   RNA: detected in 32 tissues
Protein: detected in 57 cell types		   RNA: detected in 32 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:115   1:80   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419998)		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 40, 38.9, 28.2, 22.7, 22.4   40, 38.9   40, 38.9   40, 38.9, 28.2, 22.4, 10  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA003342

  

Antibody HPA030411

  

Antibody HPA030412

  

Antibody CAB005103

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 139   75   71    

Antigen sequence

 
ITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQVI
ILYSGDKIYDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGTYQCK
VKKAPGVANKKIHLVVLVKPSGARCYVDGSEEIGSDFKI
  
EVHHDIREDVPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQV
PSEDFERTPQSPTLPPAKVAAPNLS
  
KEGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISVKNASSEYSGTYSCTV
RNRVGSDQCLLRLNVVPPSNK
  

											 
																				

																		

										
											
Matching transcripts

												
																					CXADR-001 - ENSP00000284878 [100%] 
CXADR-002 - ENSP00000383033 [100%] 
CXADR-003 - ENSP00000383030 [100%] 
CXADR-004 - ENSP00000383029 [85%] 

											 
																						CXADR-001 - ENSP00000284878 [100%] 
CXADR-002 - ENSP00000383033 [92%] 

											 
																						CXADR-001 - ENSP00000284878 [100%] 
CXADR-002 - ENSP00000383033 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					AC136352.5 - ENSG00000277666 [80%]

											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CXADR-001
CXADR-002
CXADR-003
CXADR-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003342

												
										 
																				
											
											
											
Antibody HPA030411

												
										 
																				
											
											
											
Antibody HPA030412

												
										 
																				
											
											
											
Antibody CAB005103

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Antibody-based protein profiling of the human chromosome 21

	Uhlén M et al

	Mol Cell Proteomics 2012;11(3):M111.013458

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Anti-cancer effects of REIC/Dkk-3-encoding adenoviral vector for the treatment of non-small cell lung cancer

	Shien K et al

	PLoS One 2014;9(2):e87900

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																					
											 
																							Podocyte-Specific Deletion of Murine CXADR Does Not Impair Podocyte Development, Function or Stress Response

	Schell C et al

	PLoS One 2015;10(6):e0129424

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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