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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, plasma membrane & vesicles.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:97
1:167
1:20
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035707
Antibody HPA069673
Antibody CAB009932
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1400
1:350
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035707
Antibody HPA069673
Antibody CAB009932
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
106, 18.6
106
106, 18.6
Antibody dilution
1:250
1:560
1:500
PROTEIN ARRAY
Antibody HPA035707
Antibody HPA069673
Antibody CAB009932
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
